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rabbit anti cleaved caspase3 antibody  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit anti cleaved caspase3 antibody
    Rabbit Anti Cleaved Caspase3 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cleaved+caspase3+antibody/pmc13008000-73-50-57
    Average 86 stars, based on 1 article reviews
    rabbit anti cleaved caspase3 antibody - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Incubation:

    Article Title: DMF, an activator of Nrf2, could mitigate cholic-acid-induced liver damage in high-fat diet
    Article Snippet: Immunohistochemical staining was performed on 4 μm formalin-fixed and paraffin-embedded sections using the Leica Bond MAX system (Leica Biosystems Newcastle Ltd, UK). .. Slides were baked at 60°C, dewaxed, and pretreated with epitope-retrieval solution (ER1, Leica Biosystems Newcastle Ltd, UK), followed by incubation for 30 minutes with either F4/80 antibody (1:200 70076 by Cell-Signaling) or cleaved-Caspase3 antibody (1:1,200 9661 by Cell-Signaling). .. Detection performed using the Leica Bond Polymer Refine HRP kit (Leica Biosystems Newcastle Ltd, UK).

    Article Title: Empagliflozin improves renal injury of diabetic nephropathy complicated with hyperuricemia through AMPK by promoting autophagy and inhibiting apoptosis.
    Article Snippet: .. After washing with phosphate buffer saline (PBS), the tissue sections were encircled with an immunohistochemical pen, then incubated with 5% bovine serum albumin (BSA) for 30 min. After absorbing excess serum, sections were treated with primary antibody LC3 antibody (1:500; 12,741; Cell Singling Technology (CST); USA), Cleaved caspase3 antibody (1:2000; 9664; CST; USA). .. After washing with PBS for 3 times, secondary antibodies (HRP goat anti-rabbit (cat.no.5220–0336; SeraCare; USA), HRP goat anti-mouse from Seracare (cat. no.5220–0341); 1:100; SeraCare; USA) were applied, and diaminobenzidine (DAB) was used for color development.

    Article Title: Breaking the heavy-atom paradigm: weak-donor-engineered triplet harvesting in BODIPY photosensitizers for immunogenic pyroptosis therapy
    Article Snippet: .. The membranes were blocked with 5% w/v skim 365 milk and incubated overnight at 4 ̊C with primary antibody: GSDME (Abcam, catalog no. ab215191), 366 cleaved-caspase3 antibody (Cell Signaling Technology, catalog no. 9661s), IL-1β (GeneTex, catalog 367 no. GTX74034) and β-actin (Santa Cruz Biotechnology, catalog no. sc-47778) at a 1:1,000 dilution. .. 22 368 After washing with PBST, the membranes were incubated for 2 h with the relevant secondary antibody 369 at a 1:1,000 dilution: anti-mouse (GeneTex, catalog no. GTX213110-01); anti-rabbit (Santa Cruz 370 Biotechnology, catalog no. sc-2357).

    Article Title: Overexpression of BNIP3 in renal carcinoma cells can promote apoptosis of renal carcinoma cells through HIF-1α-BNIP3-mediated autophagy
    Article Snippet: A 30 μg protein sample was taken and denatured in a boiling water bath and uploaded, proteins were separated by 10% SDS-PAGE and transferred to a PVDF membrane (ISEQ00010, Sigma-Aldrich, USA), and blocked with 5% skimmed milk for 1h at room temperature. .. The BNIP3 antibody (1:1000, A19593, ABclonal, USA), HIF-1α antibody (1:1000, A7684, ABclonal, USA), caspase3 antibody (1:500, 9662, Cell Signaling Technology, USA), cleaved caspase3 antibody (1:1000, 9661, Cell Signaling Technology, USA), Bax antibody (1:1000, A0207, ABclonal, USA), LC3B antibody (1:1000, A19665, ABclonal, USA), p62 antibody (1:1000, A19700, ABclonal, USA), and β-actin antibody (1:50000, AC026, ABclonal, USA) were incubated at 4°C overnight. .. The membrane was washed 3 times with TBST, incubated with HRP-labeled secondary antibody (1:7000, S0001, Affbiotech, Jiangsu, China) for 1h at room temperature, washed 3 times with TBST, and developed dropwise with a chemiluminescent solution.

    Article Title: Porphyromonas gingivalis secreted factors drive epithelial–mesenchymal transition (EMT) through gingipains and an H 2 S-mediated bacterial defense system
    Article Snippet: .. The next day, the cells were incubated with several concentrations of Pg -CFS or Wilkins media for 24 h. The cells were harvested and subjected to WB using the Cleaved Caspase3 antibody (Cell Signaling). .. Band quantification was established using ImageJ software.

    Article Title: Supplementary Information Appendix for: Lutetium Texaphyrin: A Photocatalyst that Triggers Pyroptosis via Biomolecular Photoredox Catalysis
    Article Snippet: .. The membranes were incubated in 5% (w/v) skim milk in PBST buffer [100 mM NaCl, 10 mM Tris-HCl (pH 7.6), and 0.1% (v/v) Tween-20] at room temperature for 1 h, and further incubated overnight at 4 ̊C in the presence of the primary antibody: cleaved-caspase3 antibody (Cell Signaling Technology, catalogue no. 9661s), caspase3 (Cell Signaling Technology, Cat# 9662s), caspase-1 (Abcam, catalogue no. ab179515), GSDME (Abcam, catalogue no. ab215191), GSDMD (Abcam, catalogue no. ab209854), and β-actin (Santa Cruz Biotechnology, catalogue no. sc-47778) at a 1:1,000 dilution. .. The membranes were then washed with PBST and incubated the next day with the relevant secondary antibody at a 1:1,000 dilution: anti-mouse (GeneTex, catalogue no. GTX213110-01); anti-rabbit (Santa Cruz Biotechnology, catalogue no. sc-2357).

    Article Title: Empagliflozin improves renal injury of diabetic nephropathy complicated with hyperuricemia through AMPK by promoting autophagy and inhibiting apoptosis
    Article Snippet: .. After washing with phosphate buffer saline (PBS), the tissue sections were encircled with an immunohistochemical pen, then incubated with 5% bovine serum albumin (BSA) for 30 min. After absorbing excess serum, sections were treated with primary antibody LC3 antibody (1:500; 12,741; Cell Singling Technology (CST); USA), Cleaved caspase3 antibody (1:2000; 9664; CST; USA). .. After washing with PBS for 3 times, secondary antibodies (HRP goat anti-rabbit (cat.no.5220–0336; SeraCare; USA), HRP goat anti-mouse from Seracare (cat.no.5220–0341); 1:100; SeraCare; USA) were applied, and diaminobenzidine (DAB) was used for color development.

    Saline:

    Article Title: Empagliflozin improves renal injury of diabetic nephropathy complicated with hyperuricemia through AMPK by promoting autophagy and inhibiting apoptosis.
    Article Snippet: .. After washing with phosphate buffer saline (PBS), the tissue sections were encircled with an immunohistochemical pen, then incubated with 5% bovine serum albumin (BSA) for 30 min. After absorbing excess serum, sections were treated with primary antibody LC3 antibody (1:500; 12,741; Cell Singling Technology (CST); USA), Cleaved caspase3 antibody (1:2000; 9664; CST; USA). .. After washing with PBS for 3 times, secondary antibodies (HRP goat anti-rabbit (cat.no.5220–0336; SeraCare; USA), HRP goat anti-mouse from Seracare (cat. no.5220–0341); 1:100; SeraCare; USA) were applied, and diaminobenzidine (DAB) was used for color development.

    Article Title: Empagliflozin improves renal injury of diabetic nephropathy complicated with hyperuricemia through AMPK by promoting autophagy and inhibiting apoptosis
    Article Snippet: .. After washing with phosphate buffer saline (PBS), the tissue sections were encircled with an immunohistochemical pen, then incubated with 5% bovine serum albumin (BSA) for 30 min. After absorbing excess serum, sections were treated with primary antibody LC3 antibody (1:500; 12,741; Cell Singling Technology (CST); USA), Cleaved caspase3 antibody (1:2000; 9664; CST; USA). .. After washing with PBS for 3 times, secondary antibodies (HRP goat anti-rabbit (cat.no.5220–0336; SeraCare; USA), HRP goat anti-mouse from Seracare (cat.no.5220–0341); 1:100; SeraCare; USA) were applied, and diaminobenzidine (DAB) was used for color development.

    Immunohistochemical staining:

    Article Title: Empagliflozin improves renal injury of diabetic nephropathy complicated with hyperuricemia through AMPK by promoting autophagy and inhibiting apoptosis.
    Article Snippet: .. After washing with phosphate buffer saline (PBS), the tissue sections were encircled with an immunohistochemical pen, then incubated with 5% bovine serum albumin (BSA) for 30 min. After absorbing excess serum, sections were treated with primary antibody LC3 antibody (1:500; 12,741; Cell Singling Technology (CST); USA), Cleaved caspase3 antibody (1:2000; 9664; CST; USA). .. After washing with PBS for 3 times, secondary antibodies (HRP goat anti-rabbit (cat.no.5220–0336; SeraCare; USA), HRP goat anti-mouse from Seracare (cat. no.5220–0341); 1:100; SeraCare; USA) were applied, and diaminobenzidine (DAB) was used for color development.

    Article Title: Empagliflozin improves renal injury of diabetic nephropathy complicated with hyperuricemia through AMPK by promoting autophagy and inhibiting apoptosis
    Article Snippet: .. After washing with phosphate buffer saline (PBS), the tissue sections were encircled with an immunohistochemical pen, then incubated with 5% bovine serum albumin (BSA) for 30 min. After absorbing excess serum, sections were treated with primary antibody LC3 antibody (1:500; 12,741; Cell Singling Technology (CST); USA), Cleaved caspase3 antibody (1:2000; 9664; CST; USA). .. After washing with PBS for 3 times, secondary antibodies (HRP goat anti-rabbit (cat.no.5220–0336; SeraCare; USA), HRP goat anti-mouse from Seracare (cat.no.5220–0341); 1:100; SeraCare; USA) were applied, and diaminobenzidine (DAB) was used for color development.

    Western Blot:

    Article Title: Porphyromonas gingivalis secreted factors drive epithelial–mesenchymal transition (EMT) through gingipains and an H 2 S-mediated bacterial defense system
    Article Snippet: .. The next day, the cells were incubated with several concentrations of Pg -CFS or Wilkins media for 24 h. The cells were harvested and subjected to WB using the Cleaved Caspase3 antibody (Cell Signaling). .. Band quantification was established using ImageJ software.

    other:

    Article Title: Methionine Restriction Attenuates Scar Formation in Fibroblasts Derived from Patients with Post-Burn Hypertrophic Scar
    Article Snippet: Cleaved caspase3 , Rabbit , 1:1000 , Cell Signaling Technology (9661S).



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    Cell Signaling Technology Inc cleaved caspase3 asp175
    (A) Schematic diagram illustrating the genomic organisation of the Dyrk1a tm1Jdc/J allele ( ENSMUSG00000022897 , Chr16:94570010 – 94695517 bp). The gene comprises 12 coding exons (black boxes) and gives rise to six mRNA isoforms. The longest transcripts ( ENSMUST00000023614 , ENSMUST00000119878, ENSMUST00000122284) encode a protein of approximately 90 kDa. LoxP sites (yellow triangles) flank exons 5 and 6 and facilitate Cre -mediated recombination of the kinase domain in the translated protein. Oligonucleotides used to assess the extent of allele recombination were designed to amplify across reference exon 4 (R4F and R4R, black arrows) and target exon 6 (T6F and T6R, red arrows). (B – E) Representative lineage tracing analysis of Dyrk1a +/f :ROSA26R R/R (B), Lhx2-Cre:ROSA26R R/R (C), Lhx2-Cre:Dyrk1a +/f :ROSA26R R/R (D) and Lhx2-Cre:Dyrk1a f/f :ROSA26R R/R (E) at P0. Robust β-galactosidase activity is detected throughout the retina of all animals that carry the Lhx2-Cre transgene (C – E) confirming Cre -mediated recombination of the ROSA26R R/R reporter allele. (F) Quantitative analysis of Dyrk1a tm1Jdc/J allele recombination efficiency in genomic DNA harvested from the retina of Lhx2-Cre ( n = 4), Lhx2-Cre:Dyrk1a +/f ( n = 4) and Lhx2-Cre:Dyrk1a f/f ( n = 4) mice at P0. A significant genotype-dependent loss of the Dyrk1a LoxP region was observed in heterozygous and homozygous animals, respectively. Quantification is based on the level of product generated by the LoxP target primer pair normalised against the level of product amplified by the reference primer pair for each biological replicate. (G) Representative immunoblots showing the main 90 kDa Dyrk1a band and the 19/17 kDa cleaved <t>Caspase3</t> bands with β-actin as a normalisation control in soluble protein extracts prepared from the retina and pigment epithelium of Lhx2-Cre , Lhx2-Cre:Dyrk1a +/f and Lhx2-Cre:Dyrk1a f/f mice at P0. (H) Quantitative analysis of Dyrk1a levels in soluble protein extracts prepared from the retina and pigment epithelium of Lhx2-Cre ( n = 12), Lhx2-Cre:Dyrk1a +/f ( n = 12) and Lhx2-Cre:Dyrk1a f/f ( n = 12) mice at P0. Dyrk1a levels were significantly reduced in a genotype-dependent manner in heterozygous and homozygous animals. Quantification is based on the 90 kDa Dyrk1a protein band normalised to β-actin within the same biological replicate. (I) Quantitative analysis of cleaved Caspase3 levels in soluble protein extracts prepared from the retina and pigment epithelium of Lhx2-Cre ( n = 12), Lhx2-Cre:Dyrk1a +/f ( n = 12) and Lhx2-Cre:Dyrk1a f/f ( n = 12) mice at P0. A genotype-dependent increase in cleaved Caspase3 levels was observed in heterozygous mice with significance reached in homozygous animals. Quantification is based on the 19/17 kDa cleaved Caspase3 protein band normalised to β-actin in the same biological replicate. All data represents the mean ± SEM values. Statistical differences were calculated using one-way ANOVA followed by a Tukey’s multiple comparison test. p-values are denoted as follows: ***p ≤ 0.001 and ****p ≤ 0.0001. Scale bar: (B – E) 50 μm. Abbreviations: bp, base pair; Casp3, cleaved Caspase3; Ch16, chromosome 16; GCL, ganglion cell layer; INBL, inner neuroblastic layer; ONBL, outer neuroblastic layer; R, reference primer pair; RPE, retinal pigment epithelium; T, target primer pair.
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    Cell Signaling Technology Inc cleaved caspase3
    (A) Schematic diagram illustrating the genomic organisation of the Dyrk1a tm1Jdc/J allele ( ENSMUSG00000022897 , Chr16:94570010 – 94695517 bp). The gene comprises 12 coding exons (black boxes) and gives rise to six mRNA isoforms. The longest transcripts ( ENSMUST00000023614 , ENSMUST00000119878, ENSMUST00000122284) encode a protein of approximately 90 kDa. LoxP sites (yellow triangles) flank exons 5 and 6 and facilitate Cre -mediated recombination of the kinase domain in the translated protein. Oligonucleotides used to assess the extent of allele recombination were designed to amplify across reference exon 4 (R4F and R4R, black arrows) and target exon 6 (T6F and T6R, red arrows). (B – E) Representative lineage tracing analysis of Dyrk1a +/f :ROSA26R R/R (B), Lhx2-Cre:ROSA26R R/R (C), Lhx2-Cre:Dyrk1a +/f :ROSA26R R/R (D) and Lhx2-Cre:Dyrk1a f/f :ROSA26R R/R (E) at P0. Robust β-galactosidase activity is detected throughout the retina of all animals that carry the Lhx2-Cre transgene (C – E) confirming Cre -mediated recombination of the ROSA26R R/R reporter allele. (F) Quantitative analysis of Dyrk1a tm1Jdc/J allele recombination efficiency in genomic DNA harvested from the retina of Lhx2-Cre ( n = 4), Lhx2-Cre:Dyrk1a +/f ( n = 4) and Lhx2-Cre:Dyrk1a f/f ( n = 4) mice at P0. A significant genotype-dependent loss of the Dyrk1a LoxP region was observed in heterozygous and homozygous animals, respectively. Quantification is based on the level of product generated by the LoxP target primer pair normalised against the level of product amplified by the reference primer pair for each biological replicate. (G) Representative immunoblots showing the main 90 kDa Dyrk1a band and the 19/17 kDa cleaved <t>Caspase3</t> bands with β-actin as a normalisation control in soluble protein extracts prepared from the retina and pigment epithelium of Lhx2-Cre , Lhx2-Cre:Dyrk1a +/f and Lhx2-Cre:Dyrk1a f/f mice at P0. (H) Quantitative analysis of Dyrk1a levels in soluble protein extracts prepared from the retina and pigment epithelium of Lhx2-Cre ( n = 12), Lhx2-Cre:Dyrk1a +/f ( n = 12) and Lhx2-Cre:Dyrk1a f/f ( n = 12) mice at P0. Dyrk1a levels were significantly reduced in a genotype-dependent manner in heterozygous and homozygous animals. Quantification is based on the 90 kDa Dyrk1a protein band normalised to β-actin within the same biological replicate. (I) Quantitative analysis of cleaved Caspase3 levels in soluble protein extracts prepared from the retina and pigment epithelium of Lhx2-Cre ( n = 12), Lhx2-Cre:Dyrk1a +/f ( n = 12) and Lhx2-Cre:Dyrk1a f/f ( n = 12) mice at P0. A genotype-dependent increase in cleaved Caspase3 levels was observed in heterozygous mice with significance reached in homozygous animals. Quantification is based on the 19/17 kDa cleaved Caspase3 protein band normalised to β-actin in the same biological replicate. All data represents the mean ± SEM values. Statistical differences were calculated using one-way ANOVA followed by a Tukey’s multiple comparison test. p-values are denoted as follows: ***p ≤ 0.001 and ****p ≤ 0.0001. Scale bar: (B – E) 50 μm. Abbreviations: bp, base pair; Casp3, cleaved Caspase3; Ch16, chromosome 16; GCL, ganglion cell layer; INBL, inner neuroblastic layer; ONBL, outer neuroblastic layer; R, reference primer pair; RPE, retinal pigment epithelium; T, target primer pair.
    Cleaved Caspase3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    (A) Schematic diagram illustrating the genomic organisation of the Dyrk1a tm1Jdc/J allele ( ENSMUSG00000022897 , Chr16:94570010 – 94695517 bp). The gene comprises 12 coding exons (black boxes) and gives rise to six mRNA isoforms. The longest transcripts ( ENSMUST00000023614 , ENSMUST00000119878, ENSMUST00000122284) encode a protein of approximately 90 kDa. LoxP sites (yellow triangles) flank exons 5 and 6 and facilitate Cre -mediated recombination of the kinase domain in the translated protein. Oligonucleotides used to assess the extent of allele recombination were designed to amplify across reference exon 4 (R4F and R4R, black arrows) and target exon 6 (T6F and T6R, red arrows). (B – E) Representative lineage tracing analysis of Dyrk1a +/f :ROSA26R R/R (B), Lhx2-Cre:ROSA26R R/R (C), Lhx2-Cre:Dyrk1a +/f :ROSA26R R/R (D) and Lhx2-Cre:Dyrk1a f/f :ROSA26R R/R (E) at P0. Robust β-galactosidase activity is detected throughout the retina of all animals that carry the Lhx2-Cre transgene (C – E) confirming Cre -mediated recombination of the ROSA26R R/R reporter allele. (F) Quantitative analysis of Dyrk1a tm1Jdc/J allele recombination efficiency in genomic DNA harvested from the retina of Lhx2-Cre ( n = 4), Lhx2-Cre:Dyrk1a +/f ( n = 4) and Lhx2-Cre:Dyrk1a f/f ( n = 4) mice at P0. A significant genotype-dependent loss of the Dyrk1a LoxP region was observed in heterozygous and homozygous animals, respectively. Quantification is based on the level of product generated by the LoxP target primer pair normalised against the level of product amplified by the reference primer pair for each biological replicate. (G) Representative immunoblots showing the main 90 kDa Dyrk1a band and the 19/17 kDa cleaved Caspase3 bands with β-actin as a normalisation control in soluble protein extracts prepared from the retina and pigment epithelium of Lhx2-Cre , Lhx2-Cre:Dyrk1a +/f and Lhx2-Cre:Dyrk1a f/f mice at P0. (H) Quantitative analysis of Dyrk1a levels in soluble protein extracts prepared from the retina and pigment epithelium of Lhx2-Cre ( n = 12), Lhx2-Cre:Dyrk1a +/f ( n = 12) and Lhx2-Cre:Dyrk1a f/f ( n = 12) mice at P0. Dyrk1a levels were significantly reduced in a genotype-dependent manner in heterozygous and homozygous animals. Quantification is based on the 90 kDa Dyrk1a protein band normalised to β-actin within the same biological replicate. (I) Quantitative analysis of cleaved Caspase3 levels in soluble protein extracts prepared from the retina and pigment epithelium of Lhx2-Cre ( n = 12), Lhx2-Cre:Dyrk1a +/f ( n = 12) and Lhx2-Cre:Dyrk1a f/f ( n = 12) mice at P0. A genotype-dependent increase in cleaved Caspase3 levels was observed in heterozygous mice with significance reached in homozygous animals. Quantification is based on the 19/17 kDa cleaved Caspase3 protein band normalised to β-actin in the same biological replicate. All data represents the mean ± SEM values. Statistical differences were calculated using one-way ANOVA followed by a Tukey’s multiple comparison test. p-values are denoted as follows: ***p ≤ 0.001 and ****p ≤ 0.0001. Scale bar: (B – E) 50 μm. Abbreviations: bp, base pair; Casp3, cleaved Caspase3; Ch16, chromosome 16; GCL, ganglion cell layer; INBL, inner neuroblastic layer; ONBL, outer neuroblastic layer; R, reference primer pair; RPE, retinal pigment epithelium; T, target primer pair.

    Journal: bioRxiv

    Article Title: Dyrk1a gene dosage controls bipolar cell development and retinal connectivity

    doi: 10.64898/2026.03.15.710015

    Figure Lengend Snippet: (A) Schematic diagram illustrating the genomic organisation of the Dyrk1a tm1Jdc/J allele ( ENSMUSG00000022897 , Chr16:94570010 – 94695517 bp). The gene comprises 12 coding exons (black boxes) and gives rise to six mRNA isoforms. The longest transcripts ( ENSMUST00000023614 , ENSMUST00000119878, ENSMUST00000122284) encode a protein of approximately 90 kDa. LoxP sites (yellow triangles) flank exons 5 and 6 and facilitate Cre -mediated recombination of the kinase domain in the translated protein. Oligonucleotides used to assess the extent of allele recombination were designed to amplify across reference exon 4 (R4F and R4R, black arrows) and target exon 6 (T6F and T6R, red arrows). (B – E) Representative lineage tracing analysis of Dyrk1a +/f :ROSA26R R/R (B), Lhx2-Cre:ROSA26R R/R (C), Lhx2-Cre:Dyrk1a +/f :ROSA26R R/R (D) and Lhx2-Cre:Dyrk1a f/f :ROSA26R R/R (E) at P0. Robust β-galactosidase activity is detected throughout the retina of all animals that carry the Lhx2-Cre transgene (C – E) confirming Cre -mediated recombination of the ROSA26R R/R reporter allele. (F) Quantitative analysis of Dyrk1a tm1Jdc/J allele recombination efficiency in genomic DNA harvested from the retina of Lhx2-Cre ( n = 4), Lhx2-Cre:Dyrk1a +/f ( n = 4) and Lhx2-Cre:Dyrk1a f/f ( n = 4) mice at P0. A significant genotype-dependent loss of the Dyrk1a LoxP region was observed in heterozygous and homozygous animals, respectively. Quantification is based on the level of product generated by the LoxP target primer pair normalised against the level of product amplified by the reference primer pair for each biological replicate. (G) Representative immunoblots showing the main 90 kDa Dyrk1a band and the 19/17 kDa cleaved Caspase3 bands with β-actin as a normalisation control in soluble protein extracts prepared from the retina and pigment epithelium of Lhx2-Cre , Lhx2-Cre:Dyrk1a +/f and Lhx2-Cre:Dyrk1a f/f mice at P0. (H) Quantitative analysis of Dyrk1a levels in soluble protein extracts prepared from the retina and pigment epithelium of Lhx2-Cre ( n = 12), Lhx2-Cre:Dyrk1a +/f ( n = 12) and Lhx2-Cre:Dyrk1a f/f ( n = 12) mice at P0. Dyrk1a levels were significantly reduced in a genotype-dependent manner in heterozygous and homozygous animals. Quantification is based on the 90 kDa Dyrk1a protein band normalised to β-actin within the same biological replicate. (I) Quantitative analysis of cleaved Caspase3 levels in soluble protein extracts prepared from the retina and pigment epithelium of Lhx2-Cre ( n = 12), Lhx2-Cre:Dyrk1a +/f ( n = 12) and Lhx2-Cre:Dyrk1a f/f ( n = 12) mice at P0. A genotype-dependent increase in cleaved Caspase3 levels was observed in heterozygous mice with significance reached in homozygous animals. Quantification is based on the 19/17 kDa cleaved Caspase3 protein band normalised to β-actin in the same biological replicate. All data represents the mean ± SEM values. Statistical differences were calculated using one-way ANOVA followed by a Tukey’s multiple comparison test. p-values are denoted as follows: ***p ≤ 0.001 and ****p ≤ 0.0001. Scale bar: (B – E) 50 μm. Abbreviations: bp, base pair; Casp3, cleaved Caspase3; Ch16, chromosome 16; GCL, ganglion cell layer; INBL, inner neuroblastic layer; ONBL, outer neuroblastic layer; R, reference primer pair; RPE, retinal pigment epithelium; T, target primer pair.

    Article Snippet: The following primary antibodies and dilutions were used: β-actin (1:20000, Sigma Aldrich, #A3854); cleaved Caspase3 (Asp175) (1:1000, Cell Signalling, #9661S); Dyrk1a (1:1000, Abnova, #H00001859).

    Techniques: Activity Assay, Generated, Amplification, Western Blot, Control, Comparison

    (A – C) Representative coronal sections taken from the retina of Lhx2-Cre (A), Lhx2-Cre:Dyrk1a +/f (B) and Lhx2-Cre:Dyrk1a f/f (C) at E14.5 to illustrate the presence of Isl1/2 + neurons and cleaved Caspase3 + apoptotic cells (A – C, arrowheads in inset images). (D – F) Representative coronal sections taken from the retina of Lhx2-Cre (D), Lhx2-Cre:Dyrk1a +/f (E) and Lhx2-Cre:Dyrk1a f/f (D) at P0 to illustrate the presence of Isl1/2 + neurons and cleaved Caspase3 + apoptotic cells (D – F, arrowheads in inset images). (G – H) Quantitative analysis of Lhx2-Cre ( n = 5), Lhx2-Cre:Dyrk1a +/f ( n = 5) and Lhx2-Cre:Dyrk1a f/f ( n = 5) animals at E14.5 (G) and P0 (H) demonstrates a significant reduction in the number of amacrine and RGCs in the retinas of homozygous animals at both ages. (I – J) Quantitative analysis of Lhx2-Cre ( n = 5), Lhx2-Cre:Dyrk1a +/f ( n = 5) and Lhx2-Cre:Dyrk1a f/f ( n = 5) mice at E14.5 (I) and P0 (J) demonstrates a significant increase the number of apoptotic cells in the retinas of Lhx2-Cre:Dyrk1a f/f animals at both ages. All data represents the mean ± SEM values. Statistical differences were calculated using one-way ANOVA followed by a Tukey’s multiple comparison test. p-values are denoted as follows: *p ≤ 0.05, **p ≤ 0.01 and ****p ≤ 0.0001. Scale bars: (A – F) 100 μm. Abbreviations: Casp3, cleaved Caspase3; CM, ciliary margin; D, dorsal; LE, lens; NR, neural retina; RPE, retinal pigment epithelium; V, ventral.

    Journal: bioRxiv

    Article Title: Dyrk1a gene dosage controls bipolar cell development and retinal connectivity

    doi: 10.64898/2026.03.15.710015

    Figure Lengend Snippet: (A – C) Representative coronal sections taken from the retina of Lhx2-Cre (A), Lhx2-Cre:Dyrk1a +/f (B) and Lhx2-Cre:Dyrk1a f/f (C) at E14.5 to illustrate the presence of Isl1/2 + neurons and cleaved Caspase3 + apoptotic cells (A – C, arrowheads in inset images). (D – F) Representative coronal sections taken from the retina of Lhx2-Cre (D), Lhx2-Cre:Dyrk1a +/f (E) and Lhx2-Cre:Dyrk1a f/f (D) at P0 to illustrate the presence of Isl1/2 + neurons and cleaved Caspase3 + apoptotic cells (D – F, arrowheads in inset images). (G – H) Quantitative analysis of Lhx2-Cre ( n = 5), Lhx2-Cre:Dyrk1a +/f ( n = 5) and Lhx2-Cre:Dyrk1a f/f ( n = 5) animals at E14.5 (G) and P0 (H) demonstrates a significant reduction in the number of amacrine and RGCs in the retinas of homozygous animals at both ages. (I – J) Quantitative analysis of Lhx2-Cre ( n = 5), Lhx2-Cre:Dyrk1a +/f ( n = 5) and Lhx2-Cre:Dyrk1a f/f ( n = 5) mice at E14.5 (I) and P0 (J) demonstrates a significant increase the number of apoptotic cells in the retinas of Lhx2-Cre:Dyrk1a f/f animals at both ages. All data represents the mean ± SEM values. Statistical differences were calculated using one-way ANOVA followed by a Tukey’s multiple comparison test. p-values are denoted as follows: *p ≤ 0.05, **p ≤ 0.01 and ****p ≤ 0.0001. Scale bars: (A – F) 100 μm. Abbreviations: Casp3, cleaved Caspase3; CM, ciliary margin; D, dorsal; LE, lens; NR, neural retina; RPE, retinal pigment epithelium; V, ventral.

    Article Snippet: The following primary antibodies and dilutions were used: β-actin (1:20000, Sigma Aldrich, #A3854); cleaved Caspase3 (Asp175) (1:1000, Cell Signalling, #9661S); Dyrk1a (1:1000, Abnova, #H00001859).

    Techniques: Comparison